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South African Journal of Botany
Berberis hispanica alkaloids extract induced cell death and apoptosis in human laryngeal cancer cells Hep-2
a Laboratoire de Physiologie des Organismes, Equipe de Physiopathologie cellulaire et moléculaire, Faculté des Sciences biologiques, Université des Sciences et Technologies, Houari Boumedienne, (USTHB), BP 32 El Alia, 16011 Alger, Algeria
b Laboratoire Central de Police Scientifique, Département de Sécurité Alimentaire et Environnement, 1 rue Abdelaziz Khelalfa, Châteauneuf, Ben Aknoun, Alger, Algeria
c Laboratoire de botanique médicale, Département de Pharmacie, Université d'Alger, 18 avenue Pasteur, 16100, Alger, Algeria
Received in revised form 20 March 2019 Accepted 15 April 2019 Available online xxxx
Edited by JJ Nair
Berberis hispanica alkaloids extract
Reactive oxygen species apoptosis
Berberis (Barberry) species contain an important amount of alkaloids and are commonly used in traditional med-icine. Alkaloids are preponderantly characterized by their numerous properties including anticancer activity. The aims of the present study were to investigate the effect and the mechanism of action of the Algerian Berberis hispanica alkaloids extract (BHAE) on Hep-2 laryngeal cancer cells. In this purpose, total alkaloids were extracted from the roots Hydrocortisone of the plant, then, were submitted to HPLC analysis. In order to determine the cells inhibition concentration (IC50), we used the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cells were enumerated using Trypan blue exclusion assay and viability by cell cycle analysis. The morphology was assessed by coloration with May Grunwald Giemsa (MGG). The evaluation of such stress markers as malondialdehyde (MDA), cytochrome c, p38MAPK, NF-kB, Akt, Erk 1/ 2 and p53 was assessed by colorimetric as-says and western blotting. Our results demonstrated that BHAE contains Berberine, an isoquinoline plant alkaloid. The IC50 was determined at 75 μg/mL and the treatment of Hep-2 cells with this dose showed anti-proliferative effect and increased SubG0 cell population (cells undergoing apoptosis) in treated cells (15.4%). Further, the col-oration of fixed cells showed that BHAE induced cells density and morphological changes. Moreover, our results showed that the treatment induced elevation of MDA, cytochrome c, p38MAPK and NF-kB level, but did not affect Akt and Erk 1/2 rates. We have also observed that the treatment with BHAE enhanced the expression of p53. Taken together, our results suggest that the BHAE triggered cell death by activation of apoptosis through ROS induction.
© 2019 SAAB. Published by Elsevier B.V. All rights reserved.
The last study of The International Agency for Research on Cancer (IARC) in 2012, recorded that the cancer has been ranked at the fifth po-sition of the world's 10 leading causes of death 8.2 million deaths (International Agency for Research on Cancer, World Health Organization, 2014).
In 2000, 160,000 new cases of laryngeal cancer were recorded. The most important risk factors of laryngeal cancer are alcohol consumption and tobacco (Tuyns et al., 1988). Actually, the treatments for this kind of cancer are often partial or total removal in addition to chemotherapy
Abbreviations: Akt, poly adp-ribose polymerase; Erk ½, extracellular signal–regulated kinases 1/2; NF-κB, nuclear factor-kappa B; P38MAPK, P38 mitogen-activated protein kinases; PARP, poly adp-ribose polymerase.
Corresponding author at: Department of Biology and Physiology of Organisms, Cellular and Molecular Physiopathology Group, University of Sciences and Technologies, Houari Boumedienne, BP 32 El Alia, 16011 Algiers, Algeria.
E-mail address: [email protected] (A. Boudjlida).
and radiotherapy (Sheahan, 2014). Currently, the scientific and techno-logical advances permit better patients care, but the curve of cancer progression remains upward.
Numerous proteins are implied during the development of cancer, and those represent as many therapeutics targets to slow down and/ or stop cancer progression. In recent years, special attention has been paid to several herbals ex-tracts for their chemotherapeutics effects on numerous cancer cell lines (Havelek et al., 2014; Imanshahidi and Hosseinzadeh, 2008; Shoeb et al., 2006).