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  • br Declaration of Competing Interest br No potential conflic

    2019-10-07


    Declaration of Competing Interest
    No potential conflicts of interest were disclosed.
    Acknowledgement
    The authors thank Professor Weijie Zhao at School of Pharmaceutical Science and Technology, Dalian University of Technology, for kindly providing L-02 cell line.
    Funding
    We gratefully acknowledge the financial supports from the National Natural Science Foundation of China (Grant Nos. 21475015, 21775015) and Australian Research Council (DE170100092).
    Appendix A. Supplementary material
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    [22] C. Sachpekidis, H. Goldschmidt, K. Kopka, A. Kopp-Schneider, A. Dimitrakopoulou-Strauss, Assessment of Selamectin metabolism and cellular proliferation in multiple myeloma: a first report on combined 18F-FDG and 18F-FLT PET/CT imaging, EJNMMI Res. 8 (1) (2018) 28.
    [28] M. Pirsaheb, S. Mohammadi, A. Salimi, M. Payandeh, Functionalized fluorescent carbon nanostructures for targeted imaging of cancer cells: a review, Mikrochim. Acta 186 (4) (2019) 231.
    [29] J.T. Mac, R. Vankayala, D.K. Patel, S. Wueste, B. Anvari, Erythrocyte-derived op-tical nanoprobes doped with indocyanine green-bound albumin: material char-acteristics and evaluation for cancer cell imaging, ACS Biomater. Sci. Eng. 4 (8) (2018) 3055–3062.
    Contents lists available at ScienceDirect
    Sensors and Actuators B: Chemical
    journal homepage: www.elsevier.com/locate/snb
    A folic acid-sensitive polyfluorene based “turn-off” fluorescence nanoprobe T for folate receptor overexpressed cancer cell imaging
    Susmita Roya, Anusha Gunukulab, Balaram Ghoshb, Chanchal Chakrabortya,
    a Department of Chemistry, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad, 500078, India
    b Department of Pharmacy, Birla Institute of Technology and Science (BITS) Pilani, Hyderabad Campus, Jawahar Nagar, Shameerpet Mandal, Hyderabad, 500078, India
    Keywords: Polyfluorene
    Polymer nanoparticles
    Fluorescence quenching
    Cancer cell imaging
    Folic acid 
    Herein, we have developed an efficient approach for folate receptor (FR) over expressed cancer cell targeting and imaging through non-covalent conjugation between folic acid (FA) and a polyfluorene based amphiphilic, fluorescent polymer nanoprobes (CPF) by fluorescence “turn off” mechanism. The pH dependent supramolecular complexation/decomplexation tendency of CPF nanoprobes with FA provides the effective reversible fluores-cence “turn off/turn on” of CPF fluorescence. The complete pH dependent complexation/decomplexation phe-nomena is characterized by photophysical and morphological characterization. The FESEM image study reveals the alteration of nanosphere structure of CPF to interconnected network after complexation with FA at pH 9 and consequently restoration of nanosphere morphology by decomplexation at pH 2. Again, the CPF nanoprobes exhibit 91% fluorescence quenching even in physiological pH 7.4 to reveal that the nanoprobes can be a suitable candidate for FA targeted turn-off fluorescent nanoprobes for FA pre-treated FR-positive cancer cells in vivo study. The fluorescence of CPF nanoprobes is significantly quenched in FA pre-treated FR-positive B16F10 cancer cell whereas it remains unchanged in FR-non overexpressed normal human corneal cell (HCE cells) even after FA pre-treatment. Furthermore, the present study can provide a dissimilar protocol to use a “turn off” fluorescent nanoprobe for FR-positive cancer cell imaging using free FA and fluorescent polymer nanoparticle rather than the conventional direct FA-fluorescent conjugated polymer complex.